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. 2020 Jul 2;9(7):1602. doi: 10.3390/cells9071602

Figure 5.

Figure 5

ZFP91 is a direct target of miR-640. (A): Sequence alignment of miR-640 and its target sites in 3′UTRs of ZFP91. (B): Relative luciferase activity in HUVECs transfected with control or miR-640 mimics and luciferase reporter plasmid expressing wild type (wt) or mutated 3′UTRs of ZFP91. The mutated 3′UTRs of ZFP91 have mutated miR-649 binding regions 1 or 2. Values are means ± SEM, expressed as relative light units (RLU). * p < 0.05, compared to the control mimic. # p < 0.05, compared to empty plasmid. (C and D): Levels of miR-640 and U6 RNA measured 48 h after transfection of HUVECs with biotinylated control or mi-R-640 mimics. * p < 0.05, compared to the control mimic. (E and F): Levels of ZFP91 mRNA in the pulled down materials of biotinylated control and miR-640 mimics. Values are means ± SEM, expressed as folds relative to the control mimic. * p < 0.05, compared to the control mimic. (F): Levels of ZFP91 mRNA in the supernatant of cell lysates following pull downs of biotinylated control or miR-640 mimics. Values are means ± SEM, expressed as folds relative to the control mimic. * p < 0.05, compared to the control mimic.