Figure 3.

Long-term adipogenic differentiation (LTAD) mediated release of free fatty acids (FFA) from adipocytes, and the influence of saturated fatty acids (SFA) in fat accumulation. The concentrations of three FFA: Palmitic acid, Stearic acid and Oleic acid were measured in the differentiated adipocyte by gas–liquid chromatography (A). The 4-day-old confluent adipocytes (2.5 × 10⁴ /cm²) were challenged with three different concentrations (as of 250, 25, and 2.5 µmol/l) of each of three SFA for 4 days followed by quantifying the fat accumulation by a TG assay kit (B). The asterisk (*) indicated statistical differences of fat accumulation of SFA treated adipocyte when compared to untreated control with significance level of p < 0.05.