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Long-term adipogenic differentiation mediated secretion of adiponectin and leptin in adipocytes. The ELISA-based quantification of adiponectin and leptin concentration in adipocytes as measured over the 20 days of culture. Data shown are the mean ± SD of three independent experiments performed in triplicates. The asterisk (*) indicated the statistical differences of protein concentration when compared with day 0, at the significance level of p < 0.05.
