Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 5;7(8):ofaa284. doi: 10.1093/ofid/ofaa284

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020. Published by Oxford University Press on behalf of Infectious Diseases Society of America.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 3. — Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of peripheral blood mononuclear cells (PBMCs) from high-risk and low-risk men who have sex with men (MSM) and blood bank donors (BBD). (A) Overview of the bioenergetics profile, of 1 representative BBD, used to evaluate mitochondrial respiratory function of PBMCs from MSM with high-risk sexual behavior (hr-MSM), MSM without high-risk sexual behavior (lr-MSM), and BBD. A sequential addition of specific inhibitors and uncouplers (in red) of the electron transport chain provides data of the OCR relative to the different components of mitochondrial respiratory function. (B) The normalized OCR measured at different stages of the mitochondrial respiration, ie, basal respiration, proton leakage, adenosine triphosphate (ATP) production, maximal respiration, spare respiratory capacity, and nonmitochondrial respiration in the different study groups. (C) Overview of the bioenergetics profile, of a representative BBD, used to evaluate glycolytic function of PBMCs from hr-MSM, lr-MSM and BBD. A sequential addition of specific stimulators and inhibitors (in red) of glycolysis provides data of the ECAR relative to the different components of glycolytic function. (D) The normalized ECAR measured at different stages of the glycolysis pathway, ie, glycolysis, glycolytic capacity, glycolytic reserve, and nonglycolytic acidification in the different study groups: hr-MSM, n = 34; lr-MSM, n = 22; BBD, n = 31. Significance was assessed on normalized data with multivariable linear regression, corrected for age (*, P < .05; **, P < .01; ***, P < .001; ****, P < .0001; significance after Bonferroni correction in red and bold). Data represent mean ± standard deviation. Some individuals were excluded from analysis due to insufficient cell numbers. SQRT, square root.