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. 2020 Jul 3;12(7):1781. doi: 10.3390/cancers12071781

Figure 2.

Figure 2

Figure 2

Upregulation of CXCR4 expression by EZH2. (A) Differential expression of EZH2 and CXCR4 mRNA in U87-MG, GBM8401, and DBTRG-05MG cells. The expression of EZH2 and CXCR4 was determined using qRT-PCR. (B) Western blot analysis of EZH2, CXCR4, and actin (loading control) in whole-cell lysates. (C) The effect of shEZH2 or Myc-EZH2 on mRNA expression in GBM8401, DBTRG-05MG, and U87-MG cells was evaluated using qRT-PCR. CXCR4 mRNA expression is shown relative to control. (D) Western blot analysis of EZH2 and CXCR4 in EZH2 knockdown/overexpression (OE) GBM cells. (mean ± SD, n = 3). * p < 0.05. The raw data of Western blots is shown in Figure S1.