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. Author manuscript; available in PMC: 2020 Aug 6.
Published in final edited form as: J Vis Exp. 2019 Sep 12;(151):10.3791/59368. doi: 10.3791/59368

Figure 3: Intrasplenic inoculation of hepatocytes into the liver of wild type C57BL/6J mice.

Figure 3:

The experimental design for ISPL inoculation of hepatocytes to induce HCC in C57BL/6J mice. (1) The spleen is identified and exteriorized. (2) The spleen is clipped with two medium-sized titanium clips. (3) Prepared hepatocytes are injected into the inferior pole of the spleen. (4) The inferior branch of the pedicle (inferior splenic pole vessels) is clipped with one medium-sized clip. Please click here to view a larger version of this figure.