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. Author manuscript; available in PMC: 2020 Aug 6.
Published in final edited form as: J Vis Exp. 2019 Sep 12;(151):10.3791/59368. doi: 10.3791/59368

Materials

Name Company Catalog Number Comments
Anesthesia machine VETEQUIP IMPAC6 anesthesia machine for surgery
Butterfly needle BD 8122963 Needle used for liver perfusion
C57BL/6 mice Jackson Lab 000664 mice used in prototol
Carprofen CRESCENT CHEMICAL 20402 carprofen for pain release
Cell Strainer CORNING REF 431751 Cell strainer, 70μm, for hepatocytes isolation
Centrifuge Beckman Coulter Allegra X-30R centrifuge for cell isolation
Clips Teleflex Medical REF 523700 Titanium Clips for spleen
Microscope Zeiss Primovert microscope for cell observation
Mtd2 mice N/A Gift from Dr. William A Held at roswell Park Cancer Institute in 2002, maintained in our lab
Needle BD REF 305109 BD precisionglide needle, 27G × 1/2 (0.4mm × 13mm)
Suture ETHICON J303H coated VICRYL suture
SV40 T Ag antibody Abcam ab16879 anti-SV40 T-antigen antibody for IHC
Syringe BD REF 309626 1 mL TB syringe for cell injection
Trypan blue SIGMA T 8154 Trypan blue solution for cell viability test
Wound clips Reflex reflex9, Part. No. 201-1000 stainless steel wound clips for wound close