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View full-text article in PMC

. 2020 Jul 7;11(7):663. doi: 10.3390/mi11070663

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The characterization of the 3D morphology and differentiation of patient-derived organoid epithelium grown in a PMI Chip. The angled views of the 3D epithelial layer derived from (A,B) normal, (C) CD, (D) UC, and (E) CRC organoids. The insets in the figures from A to E displays the vertical cross-cut view of each 3D epithelial layer. Various structural (F-actin, E-cadherin), functional (MUC2, Ki-67), or disease-specific markers (CEA, CD133) were highlighted by immunofluorescence staining followed by confocal microscopy. As a counterstaining, nuclei were highlighted in cyan. Bars, 100 μm. The quantitative comparisons of the expression of functional (MUC2) (F) and disease-specific markers (CEA, CD133) (G) in the normal and the patient-derived organoid epithelium in a PMI Chip. ** p < 0.01, *** p < 0.001.