Fig. 2.

Hepatic PTP1B disruption mitigates ethanol-induced injury and inflammation. Ctrl (Ptpn1^fl/fl) and KO (Ptpn1^fl/fl, Alb-Cre) female mice were used in the chronic plus binge model. Pair: control diet + maltose gavage, EtOH: ethanol diet + ethanol gavage. A) Plasma ALT presented in a bar chart as means + SEM (n = 5 pair-fed Ctrl mice and n = 6 for each of the remaining groups). **p < 0.01 Pair vs. EtOH and ^††p < 0.01 Ctrl vs. KO by one-way ANOVA with post-hoc Tukey's test. B) Hepatic mRNA of Il1b, Ccl2, Tnfa, Adgre1, and Ly6g were determined by qPCR normalized to Tbp then expressed as means + SEM (n = 3 per group). *p < 0.05 Pair vs. EtOH and ^†p < 0.05 Ctrl vs. KO by a two-tailed t-test. C) H&E-stained liver sections of Ctrl and KO mice with ethanol-induced inflammatory cell infiltration indicated by white circles. Scale bar: 100 μm. D) Confocal images of liver sections immunostained with the macrophage marker F4/80 (green) and DAPI counterstain (blue). Boxed areas are enlarged, and arrowheads indicate macrophages. Scale bar: 50 μm. E) Immunoblots of pNF-κB and NF-κB in hepatic lysates and each lane represents an independent animal. The phosphorylation level was normalized to protein expression and represented as means + SEM (right panel; n = 5 pair-fed Ctrl mice and n = 6 for each of the remaining groups). *p < 0.05 Pair vs. EtOH and ^††p < 0.01 Ctrl vs. KO by a two-tailed t-test. A.U.: arbitrary unit.