Fig. 3.

Hepatic PTP1B disruption attenuates ethanol-induced steatosis. Ctrl (Ptpn1^fl/fl) and KO (Ptpn1^fl/fl, Alb-Cre) female mice were used in the chronic plus binge model. Pair: control diet + maltose gavage, EtOH: ethanol diet + ethanol gavage. A) Hepatic triglycerides concentrations presented as means + SEM (n = 5 pair-fed Ctrl mice and n = 6 for each of the remaining groups). *p < 0.05, **p < 0.01 Pair vs. EtOH and ^†p < 0.05 Ctrl vs. KO by one-way ANOVA with post-hoc Tukey's test. B) Hepatic mRNA of Pparg, Srebf1, Fasn, Cd36, and Scd were determined by qPCR normalized to Tbp then expressed as means + SEM (n = 3 per group). *p < 0.05, **p < 0.01 Pair vs. EtOH and ^†p < 0.05, ^††p < 0.01 Ctrl vs. KO by a two-tailed t-test. A.U.: arbitrary unit. C) H&E-stained liver sections of Ctrl and KO mice demonstrating lipid accumulation and boxed areas are enlarged. Scale bar: 100 μm.