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. 2020 May 28;10(3):623–637. doi: 10.1016/j.jcmgh.2020.05.006

Figure 4.

Figure 4

Myenteric plexus appears normal in EXTEND-maintained lambs. (A–H) Representative confocal z-stacks of ileal muscle layers stained with antibodies against neuronal cell bodies (HuC/D) (magenta) and ICC (cKIT) (green) for (A–D) control and (E–H) EXTEND-maintained lambs. (I–N) Representative confocal z-stacks of ileal muscle stained with antibodies against glial cell nuclei (SOX10) (magenta) and glial cytoplasm (S100β) (green) for (I–K) control and (L–N) EXTEND-maintained lambs. (O) Myenteric neuron density was equivalent in control and EXTEND-maintained lambs. (P-U) Representative confocal z-stacks of ileal muscle stained with antibodies against neuronal cell bodies (HuC/D) (magenta) and neuronal nitric oxide synthase (nNOS) (green) for (P–R) control and (S–U) EXTEND-maintained lambs. All control images were taken from a control at 134-day gestational age. EXTEND images were taken from a lamb maintained for 27 days within the system to achieve 141 days’ gestational age. (U) Quantitative analysis of myenteric neuron density. Scale bars = 200 μm (A–H), 100 μm (I–T).