Figure 6.

Effect of cAMP pathway regulation on osteogenesis—expression of preosteoblast characteristic genes. ADSCs differentiated for 7 days on 2D TCPS or in 3D spheroids in osteogenic medium (0), optionally containing FSK–IBMX (FSK, 10 nM; IBMX, 500 nM) or SQ 22,536 (SQ, 100nM). Gene-expression analysis performed on Days 2 and 7. Presented data are mean from three experiments; each experiment was performed on cells isolated from a different donor. Error bars represent ±95% confidence interval. Statistical significance between “0” sample and FSK–IBMX and SQ at the given time point and culture method marked with asterisks: *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant.