Figure 5.

PRU induces necroptosis related cell death via RIPK3 and MLKL expression in AGS cells. (a) Western blot analysis of necroptosis protein markers RIPK3, p-RIPK3, MLKL, and p-MLKL protein expression on PRU treated AGS cells. The expression levels of phosphorylated form of RIPK3 and MLKL are represented graphically based on its densitometry. (b) Protein expression of p-RIPK3 and p-MLKL analyzed by western blot on PRU treated AGS cells with and without co-treatment with necrostatin-1 inhibitor (0.1 mM). The expression of the protein levels is represented graphically based on its densitometry. Values are given as the mean ± standard error of the mean (SEM) of three independent experiments. * p < 0.05 vs. control, ** p < 0.01 vs. control, *** p < 0.001 vs. control.