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. 2020 Jul 16;9(7):1707. doi: 10.3390/cells9071707

Figure 2.

Figure 2

Confocal microscopy analysis of SCESM spheroid. Seven-day-old SCESM spheroid was stained for the presence of the proliferation marker Ki-67 (anti-Ki-67) and spheroid nuclei were stained with 7-amino-actinomycin D (7-AAD). (a) Confocal microscope fluorescent and visible light images are presented. Green fluorescence represents Ki-67 and red fluorescence represents 7-AAD staining. Scale bars correspond to 200 μm; (b) Histograms of the fluorescence intensity across the SCESM spheroid diameter are presented; the green histogram displays Ki-67 and the red histogram displays the 7-AAD fluorescence intensity across the SCESM spheroid diameter.