Figure 6.

Gene expression analysis of (a) ACAN, (b) Col2A1, (c) Col1A1, (d) Col10A1, and (e) MMP13 for hMSCs encapsulated in MeHA hydrogels cultured in SCM or CiM and CS-MeHA hydrogels cultured in SCM, following 7 and 14 days of in vitro culture. (f) Col2A1/Col1A1 ratio of gene expression. The data were normalized to GAPDH and expressed as fold difference relative to undifferentiated hMSCs cultured on a TCPS. The results were analyzed using two-way analysis of variance (ANOVA) and are presented as mean values ± standard error of the mean, * p < 0.05, ** p < 0.01, *** p < 0.001 (n = 3 for each donor, hMSCs derived from three different donors).