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. 2020 Jun 30;9(7):1585. doi: 10.3390/cells9071585

Figure 1.

Figure 1

Characterization of Snail-MC38 stable clones. (A) Relative Snail mRNA expression in pcDNA-MC38 (Mock) and Snail-MC38 clones (clone #2 and #6: Snail 2 and Snail 6, respectively). Snail mRNA levels were normalized to GAPDH and EEF1A1. Results are shown as median with interquartile range; * p ≤ 0.05 and ** p ≤ 0.01, n = 7. (B) Representative Western blot analysis of Snail protein expression in Mock and Snail-MC38 clones. Control: recombinant human Snail. (C) Western blot analysis of E-cadherin (E-CADH), β-catenin (β-CTN), integrin β1, and β-actin in Mock and in Snail-overexpressing MC38 cells. (D) Distribution of E-CADH and actin in Mock and Snail-overexpressing MC38 cells (representative images, scale bar: 50 µm). (E) Cell proliferation rate of Mock- and Snail-MC38 clones at 72 and 96 h. Data are shown as mean ± SEM. Uncropped gels from Figure 1B,C and densitometry of Western blot results are shown in supplementary Figure S1B and Figure S1C, respectively.