Table 1.

List of Mutations Identified Different between DNA-Damage-Resistant and -Sensitive Isolates

Gene ID	Position	Reference	Variant	Type	Variant Type	Impact	Amino Acid Change	Product	Predict Function	Gene Essentiality
PF3D7_0710400	474979	TA	∗	DEL	missense_variant	moderate	–	DNA repair protein RAD14, putative	nucleotide excision repair, DNA damage recognition	high
	475135	A	G	SNP	missense_variant	moderate	Ile171Met
PF3D7_1006000	257175	TAATCTT TCCCTTT CTTTTTC TTCTTCC AATAAT TTC	T	DEL	frameshift_variant&missense_variant	high	Lys164fs	IMP1-like protein, putative	unknown function	low
PF3D7_1106000	257027	T	C	SNP	missense_variant	moderate	Asn39Asp	RuvB-like helicase 2	ATP-dependent 5′-3′ DNA helicase activity	high
PF3D7_1368800	2731444	TCGGTG GTCCAT TTTTTT CAACTT CCAGGT GGTCA TTTGTTT GAACTT CCAGAT GGTT	∗	DEL	frameshift_variant&missense_variant	high	Glu1567fs	DNA repair endonuclease XPF, putative	DNA repair, nuclease activity	high
PF3D7_1429900	1178944	TGATAAAG	T,∗,TGATAATG	DEL	frameshift_variant&missense_variant	high	Asn725fs	ADP-dependent DNA helicase RecQ	ATP-dependent 3′-5′ DNA helicase activity	low
PF3D7_1455300	2260945	T	G	SNP	missense_variant	moderate	Met494Arg	conserved Plasmodium protein	mismatch repair, single-stranded DNA 5′-3′ exodeoxyribonuclease activity, flap endonuclease activity	low

Mutation type, variant type, impact, and amino acid change were predicted using SnpEff (Cingolani et al., 2012) with the latest P. falciparum 3D7 genome data from PlasmoDB. Information on gene product, predict function, and gene essentiality (Zhang et al., 2018) was extracted from PlasmoDB. Asterisks (*) represent deletion of the referene DNA.