REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Phospho-mTOR (Ser2448) (D9C2) XP® Rabbit mAb	Cell Signaling Technology	CAT: #5536; RRID: AB_10691552
Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb	Cell Signaling Technology	CAT: #9234; RRID: AB_2269803
Phospho-eEF2 (Thr56) Antibody	Cell Signaling Technology	CAT: #2331; RRID:AB_10015204
Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb	Cell Signaling Technology	CAT: #2855; RRID:AB_10695878
Anti-Puromycin Antibody, clone 12D10	Merck Millipore	CAT: MABE343; RRID:AB_2566826
Biological Samples
Human Muscle	Present article	NA
Human Muscle	Morton et al., 2016; Mitchell et al., 2014; Phillips et al., 2017	NA
Chemicals, Peptides, and Recombinant Proteins
Deuterium oxide (D, 70%)	Cambridge Isotope Laboratories, Inc.	DLM-4-70-PK; CAS#7732-18-5
Dowex – 50WX8 – Hydrogen Form	Sigma Aldrich	AC335335000
PhosStop Phosphatase Inhibitor	Roche	04906837001
cOmplete Mini EDTA-free Protease Inhibitor Cocktail	Roche	11836170001
TRIzol Reagent	ThermoFisher Scientific	CAT: 15596018
CD56 Microbeads, human	Miltenyi Biotec	CAT: 130-097-042
Lipofectamine RNAiMAX Transfection Reagent	ThermoFisher Scientific	CAT: 13778030
LONG® R3 IGF-I human	Sigma-Aldrich	CAT: I1271
Puromycin dihydrochloride	Sigma-Aldrich	CAT: P8833
SYBR Select Master Mix	Applied Biosystems	CAT: 4472920
Critical Commercial Assays
E.Z.N.A Total RNA Isolation Kit	Omega Bio-Tek	SKU: R6834-01
GeneChip WT Plus Reagent Kit	ThermoFisher Scientific	CAT: 902280
High-Capacity cDNA Reverse Transcription Kit	Applied Biosystems	CAT: 4368813
Deposited Data
Raw and analyzed data	This study	GEO: GSE154846
Oligonucleotides
esiRNA human BCAT2 (esiRNA1)	Sigma-Aldrich	CAT: EHU032991
esiRNA human NID2 (esiRNA1)	Sigma-Aldrich	CAT: EHU083781
esiRNA human FKBP1A (esiRNA1)	Sigma-Aldrich	CAT: EHU106961
esiRNA human MBNL1 (esiRNA1)	Sigma-Aldrich	CAT: EHU086561
Primer: BCAT2 Forward: GAGCTGAAGGAGATCCAGTACG	Sigma-Aldrich	N/A
Primer: BCAT2 Reverse: GAGTCATTGGTAGGGAGGCG	Sigma-Aldrich	N/A
Primer: NID2 Forward: TGGAAGCTACAGGTGTGAGTG	Sigma-Aldrich	N/A
Primer: NID2 Reverse: AGGTGGGGTGATCAAGATGCAA	Sigma-Aldrich	N/A
Primer: MBNL1 Forward: CTGCCCAATACCAGGTCAAC	Sigma-Aldrich	N/A
Primer: MBNL1 Reverse: GGGGAAGTACAGCTTGAGGA	Sigma-Aldrich	N/A
Primer: FKBP1A Forward: GGTGGAAACCATCTCCCCAG	Sigma-Aldrich	N/A
Primer: FKBP1A Reverse: TCAAGCATCCCGGTGTAGTG	Sigma-Aldrich	N/A
Software and Algorithms
ImageJ	Schneider et al., 2012	https://imagej.nih.gov
Bowtie Alignment Tool	Langmead and Salzberg, 2012	http://bowtie-bio.sourceforge.net/index.shtml
Significance Analysis of Microarrays (SAM) implemented in the R programming environment (SAMR)	Tusher et al., 2001	https://cran.r-project.org/web/packages/samr/index.html
aroma.affymetrix	Bengtsson et al., 2008	https://cran.rstudio.com/web/packages/aroma.affymetrix/index.html
MEGENA	Song and Zhang, 2015	https://rdrr.io/cran/MEGENA/
Bioconductor	Gentleman et al., 2004	https://www.bioconductor.org/
CMap-L1000v1 database	Subramanian et al., 2017	https://clue.io/
Custom CDF protocol	Timmons et al., 2018	https://www.augurprecisionmedicine.com
Ciiider	Gearing et al., 2019	www.ciiider.org
Other
GeneChip Human Transcriptome Array 2.0 and RT labeling kits	ThermoFisher Scientific	CAT: 902162
Raw and analyzed data	Timmons et al., 2018, 2019	GEO: GSE154846
Human reference genome NCBI build 38, GRCh38_82p3	Genome Reference Consortium	https://www.ncbi.nlm.nih.gov/projects/genome/assembly/grc/human/