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. 2020 Jul 3;10(7):424. doi: 10.3390/brainsci10070424

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Soluble CD163 (sCD163) is associated with M2-EVs. (A) The level of CD163 in whole-cell protein extracts (WCE) from M0, M1, and M2 cells was measured by ELISA and shown as a bar graph. (B) Soluble CD163 levels in the growth media of cells from M0, M1, and M2 cells were analyzed by ELISA and shown as a bar graph. (C) sCD163 levels in EV lysates isolated from M0, M1, and M2 cells were analyzed by ELISA and shown as a bar graph. (D) Soluble CD163 levels in the growth media of cells were analyzed by ELISA after EV isolation (EV-depleted, the remaining growth media of cells from panels B and C after EV isolations). (n = 3; * p ≤ 0.05, ** p ≤ 0.01, and **** p ≤ 0.001).