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. 2020 Jun 27;10(7):407. doi: 10.3390/brainsci10070407

Figure 4.

Figure 4

hiPSC differentiation into motor neurons at d15 and d20 from healthy control (A–H) and CMT2 patient (I–P). Immunocytochemistry was performed at d15 and d20. Nuclei were stained with DAPI (blue A,E,I,M), neurons with PGP9.5 (green C,G,K,O), and motor neurons with an Islet cocktail (red B,F,J,N). At day 15, all cells were differentiated into neurons (100% PGP9.5) and 10% were Islet positive (A–D,I–L). Five days later (day 20), the proportion of motor neurons increased to up to 80% due to a maturation process (E–H,M–P). Scale bar = 50 µm. Histograms showed the progressive maturation of cells to the motor neuron (MN) phenotype between d15 and d20 for healthy control and patient (Q) (Student T Test, n = 4 to 7, *** p < 0.001).