Table 1.
Primary antibodies used for human-induced pluripotent stem cell (hiPSC), neuron, and motor neuron characterization.
| Antibody | Company | Cat Num | Species/Type | Dilution |
|---|---|---|---|---|
| Pluripotency | ||||
| Nanog | Abcam | 130095632 | Rabbit poly IgG | 1:100 |
| Oct3/4 | Santa Cruz Biotech | sc-5279 | Mouse Mono IgG2B | 1:100 |
| Sox2 | Chemicon | AB5603 | Rabbit poly IgG | 1:100 |
| Spontaneous Differentiation in Three Germinal Layers | ||||
| Pax6 | Covance | PRB-278P | Rabbit poly IgG | 1:100 |
| αSMA | DAKO | M0851 | Mouse IgG2A | 1:500 |
| Sox17 | R&D | AF1924 | Goat IgG | 1:100 |
| Neuronal and Motor Neuronal | ||||
| Tuj1 | R&D | MAB1195 | Mouse Mono IgG | 1:500 |
| PGP9.5 | Ultraclone | Ra95101 | Rabbit poly IgG | 1:500 |
| HB = MNR2 | DSHB | 81.5C10 | Chicken | 1:100 |
| Islet1 | DSHB | 40.2D6-c | Mouse Mono IgG | 1:25 |
| Islet1/2 | DSHB | 39.4D5-c | Mouse Mono IgG | 1:25 |
| ChAT | Chemicon | AB144P | Goat IgG | 1:20 |
| Other | ||||
| Ki-67 | Leica | NCL-L-Ki67-MM1 | Mouse Mono IgG | 1:200 |