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Results of assay validation parameters of HPLC for the determination of N-acetyl-L-cysteine, lidocaine HCl and benzydamine HCl at 210 nm. The concentration (µg/mL−1) was linearly correlated.
| Parameters | N-Acetyl-L-Cysteine | Lidocaine HCl | Benzydamine HCl |
|---|---|---|---|
| Retention time (min) | 2.99 ± 0.02 | 14.63 ± 1.1 | 16.42 ± 0.04 |
| Concentration (µg/mL−1) | 1.6–17.6 | 1.6–19.2 | 2.7–32.4 |
| Correlation coefficient | 0.9973 | 0.9992 | 0.9991 |
| Regression equation | y = 7E + 07x − 65577 | y = 1E + 08x + 4195.4 | y = 2E + 08x − 24180 |
