Figure 4.

Effect of recombinant chicken chemerin (Rec Chicken Chem) on in vitro testosterone production by chicken testis explants with or without the addition of hCG and/or chicken CMKLR1 antibody. Conditioned culture media were collected, and testosterone concentrations measured by ELISA assay (A). Total RNA was extracted from testis explants, and gene expression was measured by RT-qPCR for 3βHSD, STAR, and P450scc as described in the materials and methods (B). Data are means ± SEM of 6 replicates (one replicate is representative of about two testes for each condition). Bars with different letters are significantly different (p < 0.05).