Figure 5.

Effect of recombinant chicken chemerin (Rec Chicken Chem) on MAPK ERK1/2 (A), AMPK (B), Akt (C), and MAPK P38 (D) phosphorylation in chicken testis explants incubated with or without the addition of hCG and/or chicken CMKLR1 antibody. Testis explant lysates (50 μg) were resolved by SDS-PAGE, transferred to nitrocellulose, and probed with anti-phospho-MAPK ERK1/2 and anti-ERK1/2 (A), anti-phospho-AMPK and total AMPK (B), anti-phospho-Akt and total Akt (C), and anti-MAPK P38 and P38 total (D) antibodies. Bands on the blots were quantified, and the phosphorylated protein/total protein ratio is shown. Data are means ± SEM of 6 replicates (one replicate is representative of about two testes for each condition). Bars with different letters are significantly different (p < 0.05).