Table 2.
Effect of chicken recombinant chemerin on sperm motility in fresh chicken semen. Sperm samples from individual adult roosters were treated with recombinant chicken chemerin and with/without chicken CMKLR1 antibody (CMKLR1 Ab) pretreatment. Motility parameters were assessed by using Computer Assisted Sperm Analysis (CASA). They were the percentage of motile sperm (MOT); average path velocity (VAP); straight-line velocity (VSL); curvilinear velocity (VCL). Data are represented as mean ± SEM (n = 36 experiments using one sample of semen per adult rooster).
| Parameter | Time | Control | Chemerin 50 ng/mL |
Chemerin 150 ng/mL |
Chemerin 500 ng/mL |
Chicken CMKLR1 Ab (10 mg/mL) |
Chicken CMKLR1 Ab (10 μg/mL) + Chemerin 500 ng/mL |
|---|---|---|---|---|---|---|---|
| MOT (%) | T = 5 min | 74.03 ± 4.59 | 69.17 ± 4.87 | 70.00 ± 5.63 | 72.17 ± 3.90 | 73.83 ± 3.77 | 76.83 ± 2.71 |
| T = 20 min | 68.00 ± 1.69 | 64.67 ± 1.28 | 63.33 ± 2.62 |
45.33 ± 5.62
** (p = 0.0003) |
59.67 ± 2.74 | 61.17 ± 3.11 | |
| VAP (mm/s) | T = 5 min | 74.18 ± 7.20 | 76.44 ± 4.92 | 81.34 ± 9.24 | 77.08 ± 8.35 | 80.42 ± 8.87 | 78.98 ± 6.65 |
| T = 20 min | 61.26 ± 3.71 | 76.32 ± 5.28 | 76.62 ± 9.17 |
97.62 ± 8.70
* (p = 0.016) |
72.46 ± 7.83 | 68.06 ± 6.74 | |
| VSL (mm/s) | T = 5 min | 54.97 ± 5.72 | 58.22 ± 4.23 | 60.53 ± 5.44 | 56.32 ± 5.82 | 59.62 ± 6.21 | 59.38 ± 5.52 |
| T = 20 min | 47.70 ± 5.48 | 60.72 ± 6.18 | 59.28 ± 7.32 | 70.75 ± 4.25 | 58.15 ± 7.95 | 54.70 ± 7.17 | |
| VCL (mm/s) | T = 5 min | 138.40 ± 7.30 | 140.80 ± 4.35 | 145.40 ± 10.35 | 142.10 ± 8.88 | 146.20 ± 9.96 | 145.40 ± 6.28 |
| T = 20 min | 121.90 ± 5.95 | 149.6 ± 9.78 | 144.30 ± 10.68 | 155.10 ± 7.19 | 140.30 ± 11.69 | 129.90 ± 8.72 |