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Optical photograph of the buffer flow confinement into the separation chamber: (a) buffer flow rate 20 μL/min and analyte flow rate 10 μL/min, (b) buffer flow rate 10 μL/min and analyte flow rate 10 μL/min, (c) buffer flow rate 10 μL/min and analyte flow rate 5 μL/min and (d) buffer flow rate 5 μL/min and analyte flow rate 5 μL/min.
