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. 2020 Jul 10;9(7):1667. doi: 10.3390/cells9071667

Figure 6.

Figure 6

Cardiac expression of ST2 and NRP-1 in a pressure overload (PO) rodent model. Cardiac protein ST2 (A), NPR-1 protein expression (B) and sST2 mRNA (C) in myocardium samples of controls and PO rats. Gene expression of ST2L and IL-33 (D). Correlation between ST2 and NRP-1 expression (r = 0.633, p = 0.02) (E). Effects of PO on IL-33 protein (F) and MyD88, TRAF-6 and AP-1 protein expressions (G). Scatter dot blot represents the median and minimum to a maximum range of N = 5–10 animals per group. For Western blot experiments, protein densitometry was expressed in arbitrary units (AU) once normalized to stain-free for proteins. Relative mRNA expression was expressed in arbitrary units (AU) of fold change after normalization to HPRT, β-actin and GADPH housekeeping genes. Representative blots have been displayed when appropriate. * p < 0.05 vs. control group. ST2, interleukin 1 receptor-like 1; NRP-1, neuropilin-1; IL-33, interleukin 33; MyD88, Myeloid differentiation primary response 88; TRAF-6, tumor necrosis factor receptor associated factor 6 and AP-1, activator protein 1.