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. 2020 Jul 10;12(7):1861. doi: 10.3390/cancers12071861

Figure 2.

Figure 2

4LB5 Binds to PCa Cells and Inhibits Cell Proliferation (A) Basal expression levels of whole-cell NCL protein was measured in LNCaP, PC3, and DU145 PCa cell lines and compared to MDA-MB-231 expression levels via Western blot. Uncropped blots of Figure 2A are shown in Figure S4. (B) 4LB5 binding cell surfaces was assessed by an ELISA performed after incubating cells with serial dilutions of 4LB5. ELISA data shown are representative of two independent experiments performed in quadruplicate. (C) Relative cell counts of DU145, PC3, and LNCaP cells treated with 50 nM 4LB5 or control solution for 48 h. Cell survival data are the average of five biological replicates. (D) MTS assay performed after 72 h-treatment with increasing concentrations of 4LB5. MTS data are average of two assays performed in biological triplicate. (E) Light microscopy images of PCa cells taken at 48 h post-treatment with 50 nM 4LB5 or control solution. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.