Figure 3.

Treatment with 4LB5 Reduces Mature Forms of Oncogenic MicroRNAs and alters cell survival (A) microRNA expression levels were assessed via qRT-PCR in DU145, PC3, and LNCaP cells after 48 h treatment with 50 nM 4LB5 or control solution. qRT-PCR data are the average of three independent experiments performed in at least technical duplicate. (B) Caspase 3/7 activity was assessed after 48 and 72 h of 4LB5 treatment. Caspase assay was performed in biological triplicate for each time point and averaged. (C) PARP cleavage was assessed by Western blot after PCa cell treatment with 4LB5 or control solution for 48 and 72 h. PARP Western blots are representative of at least two independent experiments. Student’s t-test (2-tailed, homoscedastic) was utilized for statistical analysis. Uncropped blots of Figure 3C are shown in Figure S4. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.