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qPCR analysis of Acid-sensing Ion Channels (ASICs) and epithelial Na+ channels (ENaCs) mRNA expression in U251 MG and A172 cells and normal astrocytes. Gene expression was normalized to the β-ACTIN, GPDH and RPL13a housekeeping genes and presented as lg of relative mRNA level ± standard error of mean (SEM) (n = 3–5).
