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. 2020 Jul 8;12(7):1837. doi: 10.3390/cancers12071837

Figure 3.

Figure 3

Mambalgin-2 effect on ASIC1a expressed in U251 MG glioma cells: (a) Desensitization time constant τdes of ASIC1a. Data are presented as mean ± SEM (n = 6); (b) Whole-cell configuration of the patch clamp technique and protocol of experiment; (c) Activation of the ASIC1a currents in U251 MG cells caused by rapid change of the extracellular solution pH from 7.4 to 5.5 (control, on the left), in the solution with pH 7.4 (in the middle), and upon application of 10 µM benzamil to the extracellular solution with pH 5.5 (on the right); (d) Activation of the ASIC1a currents in U251 MG cells caused by rapid change of the extracellular solution pH from 7.4 to 5.5 (control, on the left), in the solution with pH 7.4 (in the middle), and upon application of 1.5 µM mambalgin-2 to the extracellular solution with pH 5.5 (on the right); (e) Influence of 1.5 µM mambalgin-2 application to the extracellular solution with pH 5.5 on the peak amplitude of the ASIC1a currents in U251 MG cells. Data are presented as normalized peak current amplitude, % of control ± SEM (n = 3). Control level (100%, untreated cells) is shown by dashed line. *** (p < 0.001) indicates significant difference from the control according to two-sided t-test.