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. 2020 Jun 27;9(7):1569. doi: 10.3390/cells9071569

Figure 5.

Figure 5

Co-localisation between the TULV NP and either the endoplasmic reticulum (ER) or the Golgi compartments at the early, peak and persistent stages of TULV infection in Vero E6 cells. The spatial distribution of the endoplasmic reticulum and the Golgi network (both magenta) was observed alongside TULV NP (green) in Vero E6 cells infected with TULV at an MOI of 0.1 by confocal microscopy at 40 X magnification at (A) 36 h post infection (hpi), (B) 7 days post infection (dpi) and (C) 30 dpi. The scale bars represent 30 μm. Fluorescent line scans were taken using Fiji software with the designated number labels corresponding to the adjacent line scan plots. Nuclei were stained with DAPI (blue), TULV NP was detected using NP antisera, with the ER detected using concanavalin-A and the Golgi was detected by specific antisera. Images shown in panels A, B and C show a representative analysis of the 19, 27 and 13 cells, respectively.