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. 2020 Jul 19;9(7):2293. doi: 10.3390/jcm9072293

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Sensolyte assay for kinetic analysis of matrix metalloproteinase (MMP) activity after BMP2- or BMP4-treatment. (A) A remarkable increase (~6 fold) in the slope of substrate cleavage by MMPs was seen against time for the BMP4-treated ARPE-19 cells in comparison with BMP2-treated ARPE-19 cells. (B) MMP2 activity was significantly (P < 0.001) increased after exposure to BMP4 but not BMP2; Black circles: control; Red squares: BMP2; Red Triangles: BMP4. Results are presented as mean ± SD; n = 4–6 per group.