Figure 1.

Study workflow. Prostate glands were removed from patients with prostate cancer (PCa), through radical prostatectomy. Anticipated PCa tissue and adjacent benign tissue (ABT) were freshly frozen. Confirmative hematoxylin and eosin staining was later applied on paraffin sections, to verify the histological assignment. Metabolites were isolated from frozen, paired PCa and ABT samples, and were subjected to capillary electrophoresis (CE) and liquid chromatography (LC), coupled with mass spectrometry (MS).