Figure 3.

Experimental setup for the use of bleed chip with blood sample. Device inlet is connected to a syringe pump with a syringe filled with recalcified whole human blood. The bleeding channel is connected to a 0.5 mm polyethylene tube with graduations every 0.5 cm to record the blood flow rate through the bleeding channel. An equal length of polyethylene tubing is connected to the second outlet leading to waste collection. Real time dynamics of blood flow inside the bleed chip is captured with the 10× objective of a DIC Zeiss Axio Imager 2 microscope. Arrows denote the direction of blood flow.