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. 2020 Jul 13;12(7):1880. doi: 10.3390/cancers12071880

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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C₂C₁₂ Myotube metabolic profile identified in C₂C₁₂ cells. Concentration of (a) Aspartate; (b) Choline; (c) Dimethylamine; (d) Glucose-6-phosphate; (e) Glutamine; (f) N-Acetyllysine; (g) Sarcosine. C₂C₁₂ myotube cells were distributed into control (C); leucine supplementation medium (L); Walker Factor treatment (WF) and Walker Factor treatment and leucine supplementation medium (WFL). Data were expressed as mean ± standard deviation (SD) and analysed by one-way ANOVA followed by Tukey. (^a) p < 0.05 comparison to C; (^b) p < 0.05 comparison to L and (^c) p < 0.05 comparison to W.