Table 1.

Polymerase chain reaction cycling condition and primer sequences.

Gene	Primer Sequences	PCR Cycling Conditions	Reference
gyrB	F- GCC CTG GTA TGT ATA TTG GAT CTA C R- GGT CAT AAT AAC TTC TAC AGC AGG A	Initial denaturation of 2 min at 94 °C, followed by 30 cycles at 94 °C for 30 s at, 52 °C for 1 min and 72 °C for 30 s, and final elongation at 72 °C for 10 min.	[12]
nheABC	F- AAG CIG CTC TTC GIA TTC R- ITI GTT GAA ATA AGC TGT GG	Initial denaturation of 5 min at 94 °C, followed by 30 cycles at 94 °C for 30 s, 49 °C for 1 min and at 72 °C for 1 min, and final elongation at 72 °C for 5 min	[13]
hblABD	F-GTA AAT TAI GAT GAI CAA TTT C R- AGA ATA GGC ATT CAT AGA TT
ces	F- TTG TTG GAA TTG TCG CAG AG R-GTA AGC GGA CCT GTC TGT AAC AAC	Initial denaturation of 2 min at 94 °C, followed by 30 cycles at 94 °C for 30 s at, 52 °C for 1 min and 72 °C for 30 s and a final elongation at 72 °C for 10 min	[13]
cytK-plcR	P1- CAA AAC TCT ATG CAA TTA TGC AT P3- ACC AGT TGT ATT AAT AAC GGC AAT C		[15]
tasA	F- AGC AGC TTT AGT TGG TGG AG R-GTA ACT TAT CGC CTT GGA ATTG	Initial denaturation of 5 min at 94 °C, followed by 40 cycles at 94 °C for 30 s, 59 °C for 45 s and 72 °C for 45 s, and final elongation at 72 °C for 5 min	[11]
sipW	F- AGA TAA TTA GCA ACG CGA TCTC R- AGA AAT AGC GGA ATA ACC AAGC	Initial denaturation of 5 min at 94 °C, followed by 40 cycles at 94 °C for 30 s, 54 °C for 45 s, and 72 °C for 45 s, and a final elongation at 72 °C for 5 min