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. 2020 Jul 16;8(7):1060. doi: 10.3390/microorganisms8071060

Figure 1.

Figure 1

(a) Aa23 cell counts prior to Wolbachia isolation procedures. * above the bars represents a significant difference (t-test, p < 0.05), (b) pre- and (c) post-Wolbachia isolation using Trypan Blue stain, (d) Polymerase chain reaction (PCR) confirmation of Wolbachia infections in Aa23 cells and isolations, (e) Fluorescent in-situ hybridization (FISH) staining of Aa23 cells confirming Wolbachia infection, (f) FISH staining of Aa23-T aposymbiotic cells showing the absence of a Wolbachia infection, and (g) Baclight LIVE/DEAD™ staining of Wolbachia immediately after isolation procedures, and (h) Baclight LIVE/DEAD™ staining of heat killed Wolbachia. White scale bars represent 50 µM.