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(A) Isolated Wolbachia stained with Baclight LIVE/DEAD™ viability assays in Schneider’s medium supplemented with 10% FBS and an adjusted pH of 6.5, 6.8, 7.0, and 7.5. Live and dead Wolbachia are green and red, respectively. (B) Live Wolbachia was counted using Image-J from three representative fields of view at 100x. Significant differences are indicated by different letters above each bar (Mann–Whitney, p < 0.05).
