Table 3.
Differentiation protocol comparison. The STEMdiff and CDM3 protocols were compared to differentiate cardiomyocytes (CMs) using two hiPSC-lines, one from a healthy patient (WTSIi020-A) and another from a hypertrophic cardiomyopathy patient (UKKi025-A). In terms of cell quality, the CDM3 protocol proved to be better for the differentiation of WTSIi020-A and UKK025-A into CMs.
| WTSIi020-A | UKKi025-A | ||||||
|---|---|---|---|---|---|---|---|
| Batch No. | Protocol | Passage | Confluence | CMs Quality | Passage | Confluence | CMs Quality |
| 1st | STEMdiff | 38 | 95% | Low | 42 | 95% | Mid |
| 2nd | STEMdiff | 39 | 95% | Differentiation Failed | 41 | 95% | Mid |
| 3rd | STEMdiff | 39 | 95% | × | 41 | 95% | × |
| CDM3 | 39 | 85% | Mid | 41 | 85% | High | |
| 4th | CDM3 | 40 | 85% | High | 41 | 90% | High |
| 5th | CDM3 | 42 | 90% | High | 42 | 95% | High |