Figure 5.

The expression of metabolic markers in paired (primary-relapse) cases. The color scales represent the medians and means of different metabolic marker H-scores in primary and relapse cases (A). LDHA and G6PDH immunohistochemical staining scores of primary and pretreated RMS cases showed remarkable alterations (B). The higher expressions of LDHA and the significantly decreased expressions of G6PDH in relapsed cases suggest the increased importance of Warburg-effect instead of pentose-phosphate pathway in relapsed RMSs. DAB (brown) and hematoxylin were used as chromogen and counterstaining, respectively. The numbers in the left upper corner of the pictures refer to the given H-scores. Pictures were taken with CaseViewer 2.3 software (3DHistech Ltd., Budapest, Hungary), the scale bars show 25 µm.