Figure 3.

CN133 and CN147 induce cell death in EPN cell lines: (a) EPP and EPV cell lines were treated with 1 μM of CN133 and CN147 and apoptosis was evaluated by flow cytometry (FACS); (b,c) Graph represents the average quantification of the percentage of cells in sub-G1 of three independent experiments (n = 3/experiment) ± SEM. ** p < 0.01; *** p < 0.001; EPP (d) and EPV (e) were treated with 1 µM of CN133 and CN147 for 24 h. At the end of the treatment, cells were harvested and stained with the Annexin V kit following the manufacturer’s instruction. Graph represent the average quantification (n = 3/each). * p < 0.05; ** p < 0.01; *** p < 0.001.