Figure 6.

CN133 modulates several cancer-related pathways. Uncropped Blots are shown in Figure S9 (a) Quantitative real-time PCR (RT-qPCR) using primers for seven upregulated genes and two downregulated genes. Values are represented as fold change of treated versus non treated cells and are the mean ± SEM of three independent experiments; (b) Western blot of some upregulated genes in response to CN133; (c) gene set enrichment analysis GSEA performed on genes differentially expressed in presence or absence of CN133 treatment on EPP cell line; (d) Western blot of the proteins related to hypoxia (HIF1α) or to the unfolded protein response from EPP cells treated with 1 μM of CN133 for the indicated times. Actin was used as a loading control; (e) Western blot of proteins related to the mTOR/AKT pathway; (f) Western blot of apoptotic-related proteins; (g) schematic representation of the proposed mechanism of action of CN133.