Response of the Salmonella csgD regulatory network to changes in osmolarity. csgDEFG (A,E), csgBAC (B,F), adrA (C,G), and cpxP (D,H) expression was measured in S. typhimurium 14028 during growth at 28 °C in media premixed with 25, 50, 75, 100, 125 or 150 mM salt (A–D) or with 50, 100, or 150 mM salt added during growth (E–H; vertical line shows the time of addition at 18 h). For each graph, luminescence (light counts per second) divided by the optical density at 600 nm (Lum/OD) was plotted as a function of time with each curve representing a single growth condition. The mean and standard deviations are plotted from experiments performed in triplicate (A–C, E–H) or from a single representative experiment (D).