FIGURE 1.

MRFV genome organization and loci tested for viability as insertion sites. The genome outline shows replication‐associated proteins (MTR, PRO, NTP/HEL, and POL) processed from the replicase polyprotein (RP) at predicted cleavage junctions. The genome is encapsidated by 2 carboxyl co‐terminal coat proteins: CP1, likely cleaved from the polyprotein; and CP2, likely expressed from a subgenomic RNA. Individual loci targeted for insertion of heterologous sequences are indicated, [(a)–(d)]. The numbers straddling the arrows indicate MRFV nucleotide junctions carrying insertions. Insertion cassettes are annotated as a₁ to d₁, as well as the constructs derived from each of the cassettes (see Table 1 first column). The sequence identity of each cassette is as indicated