FIGURE 4.

Testing of the stability of MRFV‐PDS₁₂₀ using serial passage inoculations with crude plant sap. (a) MRFV‐PDS₁₂₀ symptoms and chlorophyll photobleaching phenotype at 30 dpi for each passage, compared to MRFV‐WT and noninoculated plants (HC). (b) RT‐PCR analysis (primers sm151 and sm152) of the integrity of MRFV‐PDS₁₂₀ at each of the four passages. All the MRFV‐PDS₁₂₀‐infected plants obtained for each passage were RT‐PCR assayed both at 15 dpi (not shown) and at 60 dpi (representative gel shown here). Healthy plants (HC) and water (H₂O) were included as negative controls, and MRFV‐PDS₁₂₀ plasmid (PL) as a positive control. M: 100 bp DNA marker.