FIGURE 6.

Capacity of HEL/POL junction to hold larger inserts. (a) Similar to MRFV‐PDS_120, molecular assays for MRFV‐PDS₂₃₁ used primers sm151 and 152 for RT‐PCR detection, amplifying 1,275 bp in MRFV‐PDS₂₃₁ and 963bp in MRFV‐WT. (b) Virus symptoms and chlorophyll photobleaching phenotype induced by MRFV‐PDS₂₃₁ at 30 dpi, compared to MRFV‐WT and noninoculated plants. (c) RT‐PCR analysis of virus accumulation in systemic leaves at 30 dpi. The gel shows RT‐PCR detection of MRFV‐PDS₂₃₁ and MRFV‐WT in systemic leaves of inoculated plants. Healthy plants (HC) and water (H₂O) were included as negative controls and the MRFV‐PDS₂₃₁ plasmid (PL) as a positive control. M: 100 bp DNA marker. (d) Northern blot analysis of photobleaching phenotype induced by MRFV‐PDS₂₃₁ was as described in Figure 3. Levels of PDS mRNA and siRNAs from MRFV‐PDS₂₃₁‐infected leaves is shown (lanes 1–4) compared to MRFV‐infected (lanes 5–6) and healthy plants (lanes 7–8). The relative levels of PDS mRNA were determined as described in Figure 3