Table 2.
Thickness values and fluorescence quantification (mean ± standard deviation) determined from CLSM of CaS and CaR biofilms labeled with different fluorochromes.
| Fluorochromes | Thickness (µm) | Fluorescence Intensity | ||||||
|---|---|---|---|---|---|---|---|---|
| CaS | CaR | CaS | CaR | |||||
| Contr. | NAC | Contr. | NAC | Contr. | NAC | Contr. | NAC | |
| S9 | 24.00 | 24.00 | 24.00 | 20.00 | 77.68 ± 18.47 | 45.86 ± 5.16 | 74.74 ± 9.96 | 38.85 ± 15.64 |
| PI | 13.22 ± 5.65 | 28.64 ± 6.06 | 6.04 ± 1.03 | 55.06 ± 18.52 | ||||
| Conc | 36.00 | 30.00 | 18.00 | 12.00 | 105.99 ± 15.09 | 89.22 ± 11.86 | 78.82 ± 21.11 | 40.83 ± 14.44 |
| CalcW | 34.38 | 23.04 | 34.38 | 30.56 | 100.33 ± 30.68 | 125.12 ± 27.71 | 178.29 ± 9.86 | 23.34 ± 6.91 |
| SR | 51.92 | 23.60 | 47.2 | 23.60 | 25.70 ± 7.33 | 49.16 ± 10.36 | 70.42 ± 14.40 | 56.25 ± 19.30 |
| NRp | 40.00 | 28.00 | 37.76 | 28.32 | 44.85 ± 15.70 | 37.01 ± 11.12 | 148.14 ± 12.66 | 16.51 ± 4.29 |
| NRn | 69.62 ± 21.39 | 74.75± 21.50 | 107.62 ± 8.00 | 15.59 ± 2.51 | ||||
Fluorochrome abbreviations and respective definitions: Syto 9 (S9) for live cells; Propidium Iodide (PI) for dead cells; Concanavalin (Conc) for α-D-mannosyl and α-D-glucosyl; Calcofluor White (CalcW) for β-glucans; Sypro Ruby (SR) for proteins; Nile Red for polar lipids (NRp) and Nile Red for neutral lipids (NRn). Contr.: Control (no NAC).