Figure 1.

An increased number of neuroblasts is generated after stroke by blocking Notch-signaling. Analysis of Dcx expressing cells in the brain of mice with selective ablation of Rbpj in astrocytes after stroke: (A) Summary of the experimental procedure—mice were administered tamoxifen via intraperitoneal injection for five consecutive days. One week after the last injection, stroke was induced, and the animals were then sacrificed seven weeks later; (B) Overview of Dcx⁺ cells in the injured and uninjured hemisphere seven weeks after stroke (scalebar = 500 µm); (C) Quantification of the number of Dcx⁺/Tomato⁺ cells. For each animal, four sections around the core of the lesion have been selected and quantified. The average number of cells has been compared between the injured and the uninjured hemisphere from the same animals (p = 0.041) and with the injured hemisphere of wild-type mice (p = 0.005). Data were analyzed using a paired t-test, and results are presented as mean ± SD; (D,E) Close-up of clusters; (D) Single close-up; (E) Dcx⁺/Tomato⁺ cells found in the penumbra of the stroke area. Arrowheads indicate cells that are positive for both Dcx and Tomato, while the arrow points at a Tomato+ cell (scalebar = 15 µm and 6 µm respectively in D and E).