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. 2020 Jul 20;9(7):1732. doi: 10.3390/cells9071732

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Analysis of cluster number and size. Analysis of the Ascl⁺ and Dcx⁺ clusters in the stroke side compared to the uninjured striatum: (A,B) Quantification of the total number of clusters. Groups of Ascl1⁺ cells have been defined as clusters only when found in contact with each other and in numbers ≥3 cells; Dcx⁺ cells had to be in groups ≥7 to be considered a cluster. The number of Ascl1⁺ clusters was significantly higher in the stroke side compared to the uninjured striatum (p = 0.014); (C,D) Analysis of the size of Ascl1⁺ and Dcx⁺ cells in the stroke and uninjured striatum; (E) Analysis of the total number of cells found in clusters and as singlets. Ascl1⁺/Tomato⁺ cell numbers are increased both as single cells (p = 0.022) and in clusters (p = 0.023), while there is a significant increase only in the number of Dcx⁺/Tomato⁺ neuroblasts singlets (p = 0.0097). The data were analyzed using a paired t-test and are presented as mean ± SD.